Coding
α1G

Part:BBa_K2263003:Design

Designed by: Imke Ensinck   Group: iGEM17_KU_Leuven   (2017-10-30)


α1G calcium ion channel


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1258
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4955
    Illegal BamHI site found at 344
    Illegal BamHI site found at 2118
    Illegal BamHI site found at 6777
    Illegal BamHI site found at 6879
    Illegal XhoI site found at 5174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 89
    Illegal NgoMIV site found at 2544
    Illegal NgoMIV site found at 5363
    Illegal NgoMIV site found at 6515
    Illegal NgoMIV site found at 6653
    Illegal NgoMIV site found at 6759
    Illegal AgeI site found at 4382
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1359
    Illegal BsaI site found at 4098
    Illegal BsaI.rc site found at 1198
    Illegal BsaI.rc site found at 5321
    Illegal BsaI.rc site found at 6385
    Illegal BsaI.rc site found at 6434
    Illegal SapI site found at 2619
    Illegal SapI site found at 4299


Design Notes

We had to remove several illegal restriction sites using silent mutagenesis. We also lowered the GC content to allow synthesis as gBlock gene fragments.


Source

From the a1G mRNA sequence found in Mus musculus.

References